Imaging and analysis of blood flow

(血流・血行状態のイメージングと解析)

Imaging and analysis of blood flow

                  

Tomohiro Kurata(D3),Minori Takahashi(M2) 
Kodai Nihei(B4),Ryohei Hashimoto,Sinko Ri(graduate)

Microcirculation is the small blood vessels whose diameter is less than 100 μm and is found in all parts of the body. Its functions areis gas and nutrient exchange between the blood and the tissues. It ensures adequate immunological functions. The microcirculation is markedly altered in sepsis, that these alterations are more severe in nonsurvivors than in survivors. So, the monitoring of microcirculation is needed for understanding the biological condition. Sidestream dark-field (SDF) imaging (Ince, 2005) was developed as an imaging system to observe microcirculation noninvasively. We have developed an SDF probe using three color LEDs and it produces images of three wavelength by switching the LED light sources. As a result, we verify that SDF imaging allows shooting blood flow of red blood cells (RBCs) at organs of the pig and sublingual of the human. By utilizing the difference in the spectral characteristics of oxygenated and de-oxygenated hemoglobin in the RBCs from the spectral image, we are aiming to establish a blood oxygen saturation measurement technique. Furthermore, to evaluate quantitatively the blood flow by analyzing a moving image, we are aiming to monitor the state of health.
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   Schematic illustration of the sidestream dark-field (SDF) imaging technique
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Spectral intensity of the color LED chips and spectral absorption coefficient of Hb and HbO2
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Spectral images of a pig mucosal microcirculation at the three selected wavelengths
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  (a)Example of vein vessel segment      (b)Profile lines and FWHM edges                                    overlaid vessel segment
Trace and edges found in example vessel segment

English